Li Lingxiao, He Yi, Wang Yuanyuan, et al. Adaptive optics scanning laser ophthalmoscopy with two sources[J]. Opto-Electronic Engineering, 2019, 46(2): 180137. doi: 10.12086/oee.2019.180137
Citation: Li Lingxiao, He Yi, Wang Yuanyuan, et al. Adaptive optics scanning laser ophthalmoscopy with two sources[J]. Opto-Electronic Engineering, 2019, 46(2): 180137. doi: 10.12086/oee.2019.180137

Adaptive optics scanning laser ophthalmoscopy with two sources

    Fund Project: Supported by National Science Foundation of China (61605210), National Instrumentation Program (NIP) (2012YQ120080), Jiangsu Province Science Fund for Distinguished Young Scholars (BK20060010), the Frontier Science Research Project of the Chinese Academy of Sciences (QYZDB-SSW-JSC03), the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB02060000), the National Key Research and Development Program of China (2016YFC0102500), and the Zhejiang Province Technology Program (2013C33170)
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  • Adaptive confocal laser ophthalmoscope with the high-resolution and dynamic imaging ability has been widely applied in specific biomedical and clinical medical fields. In order to apply the noncircular pupil filter and other pupil modulation technology without influence in wavefront detection, the system needs two light sources for imaging and aberration correction respectively. This paper has designed an adaptive optics scanning laser ophthalmoscopy with two sources, and then analyzed the differences of the aberration of the two light sources. Then, the aberration correction and high-resolution imaging ability of the system have been verified, and the brightness, contrast and resolution of the image have been significantly improved after close-loop. Finally, we have studied the feasibility of realizing the dark field imaging by semi-circular pupil and obtained the bright and dark field images of the artificial eye.
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  • Overview: Adaptive confocal laser ophthalmoscope with the high-resolution and dynamic imaging ability has been widely applied in specific biomedical and clinical medical fields. In order to get more information of the retina, the noncircular pupil filter and other pupil modulation technology should be applied in the adaptive confocal laser ophthalmoscope without influence in wavefront detection, so two light sources are need for imaging and aberration correction respectively. We have designed an adaptive optics scanning laser ophthalmoscopy with infrared and visible light sources. The principle of the system and some parameters of optical elements have been introduced. The two beams with different wavelengths are combined and separated by dichroic mirrors. The pupil filter could be utilized in entrance and exit of imaging optical path and it could not make an impact on the wavefront detection. Since two different light beams are used at the same time, we should consider the chromatic dispersion effect of human eye. By measuring the human eye aberrations made by the two light sources, it could be found that the biggest difference is in the defocus and the other high-order aberrations are almost same. We have calculated the difference of the defocus of the two sources by empirical formulas and finally compensated it by moving the pinhole that is in front of PMT. Then, the aberration correction and high-resolution imaging ability of the system have been verified through the experiments in human retina. Wavefronts before and after close-loop have been obtained, which proves that the system has realized the diffraction limit after the close-loop. We have found from the image that both brightness and contrast of the image have been significantly improved. In addition, the spectra of the retinal image have also showed that the intensities of almost whole the spatial frequency components are increased, so that more details could be observed. Finally, we studied the feasibility of realizing the dark field imaging by semi-circular pupil, which could block the reflected light and let some of the scattered light pass through, and thus the dark filed image can be obtained. We have inserted two semi-circular pupils in entrance and exit pupils of the adaptive optics scanning laser ophthalmoscopy and obtained the dark field image of the artificial eye. By comparing the bright and dark field images, it could be seen that the main information are different, which may help us obtain more details of the retina based on the multi-layer structure of the retina.

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