Traditional pinhole spherical wave digital in-line holography has proved to be powerful imaging tools. Image quality is affected by uncertain round of pinhole. Here, we propose a well-distributed sphere wave generation method and it demonstrates wide field of view and high resolution microscopy. The laser focuses into an infinitesimal spot through laser beam expander and microscope objective. Pinhole permutation with different sizes is utilized to match the focal point, and emerges an ideal spherical wave. Interference fringes pattern, formed by reference sphere wave and scattered sphere wave of object, is collected by large area image sensor. The influence of dirty in image sensor and parasitic light is eliminated through subtraction with and without object. Fresnel inverse transformation reconstruction algorithm presents the object information. Biology microscopy experiments demonstrate that the proposed techniques increase the flexibility in producing well-distributed point light source and improve the image quality. Field of view is 3.22 mm×3.22 mm and resolution is 5.09 μm. Furthermore, adjustable field of view with magnification, fast, no-contact make it to be a promising tool in optical element measurement, material identification, biology and medicine.
Biology microscopy using well-distributed sphere digital in-line holography
First published at:Dec 22, 2018
 Jiang M S, Zhang N N, Zhang X D, et al. Applications of hybrid search strategy in microscope autofocus[J]. Opto-Electronic Engineering, 2017, 44(7): 685–694.
江旻珊, 张楠楠, 张学典, 等. 混合搜索法在显微镜自动对焦中的应用[J]. 光电工程, 2017, 44(7): 685–694.
 Lu B B, Liu L Q, Zheng Y M, et al. A method for segmenting the microscopic cable harness image automatically[J]. Opto-Electronic Engineering, 2016, 43(10): 49–55.
芦碧波, 刘利群, 郑艳梅, 等. 一种线束端子显微图像全自动分割方法[J]. 光电工程, 2016, 43(10): 49–55.
 Garcia-Sucerquia J, Xu W B, Jericho S K, et al. Digital in-line holographic microscopy[J]. Applied Optics, 2006, 45(5): 836–850.
 Xu W, Jericho M H, Meinertzhagen I A, et al. Digital in-line holography of microspheres[J]. Applied Optics, 2002, 41(25): 5367–5375.
 Malek M, Allano D, Coëtmellec S, et al. Digital in-line holography for three-dimensional-two-components particle tracking velocimetry[J]. Measurement Science and Technology, 2004, 15(4): 699–705.
 Das B, Yelleswarapu C S. Dual plane in-line digital holographic microscopy[J]. Optics Letters, 2010, 35(20): 3426–3428.
 Zhang Y C, Xie C Q. Differential-interference-contrast digital in-line holography microscopy based on a single-optical-element[J]. Optics Letters, 2015, 40(21): 5015–5018.
 Tian P, Hua Y L, Yang F, et al. High efficiency and flexible working distance digital in-line holographic microscopy based on Fresnel zone plate[J]. Measurement Science and Technology, 2017, 28(5): 055209.
 Kim M K. Wavelength-scanning digital interference holography for optical section imaging[J]. Optics Letters, 1999, 24(23): 1693–1695.
 Zhang T, Yamaguchi I. Three-dimensional microscopy with phase-shifting digital holography[J]. Optics Letters, 1998, 23(15): 1221–1223.
 Yamaguchi I, Kato J I, Ohta S, et al. Image formation in phase-shifting digital holography and applications to microscopy[J]. Applied Optics, 2001, 40(34): 6177–6186.
 Poon T C. Recent progress in optical scanning holography[J]. Journal of Holography and Speckle, 2004, 1(1): 6–25.
 Yamaguchi I, Zhang T. Phase-shifting digital holography[J]. Optics Letters, 1997, 22(16): 1268–1270.
 Das B, Yelleswarapu C S, Rao D V G L N. Quantitative phase microscopy using dual-plane in-line digital holography[J]. Applied Optics, 2012, 51(9): 1387–1395.
 Massig J H. Digital off-axis holography with a synthetic aperture[J]. Optics Letters, 2002, 27(24): 2179–2181.
 Sánchez-Ortiga E, Doblas A, Saavedra G, et al. Off-axis digital holographic microscopy: practical design parameters for operating at diffraction limit[J]. Applied Optics, 2014, 53(10): 2058–2066.
Department of Science and Technology of Sichuan Province (2015JQ0009) and the National Natural Science Foundation of China (61705232)
Get Citation: Tian Peng, Yan Wei, Li Fanxing, et al. Biology microscopy using well-distributed sphere digital in-line holography[J]. Opto-Electronic Engineering, 2019, 46(1): 180110.
Previous: Multi-exposure image fusion based on tensor decomposition and convolution sparse representation